000190536 001__ 190536
000190536 005__ 20180913062139.0
000190536 037__ $$aCONF
000190536 245__ $$aSelf-assembled melamine microlens arrays for immunofluorescence enhancement
000190536 269__ $$a2013
000190536 260__ $$c2013
000190536 336__ $$aConference Papers
000190536 520__ $$aSelf-assembled dielectric microlenses are used for focusing of the fluorescent signal emitted from a surface-based immunoassay, performed on silane micropatterns as assay substrates, to enhance the detection signal. In our model system, a fluorescent immunocomplex is formed on (3-aminopropyl)triethoxysilane (APTES) microstructures, and then carboxyl-functionalized melamine microparticles with two different sizes are electrostatically self-assembled on the microstructures. Mouse IgG diluted in phosphate buffered saline (PBS) is used as model target antigen and easily detected down to a concentration of 2 ng/mL. We also present a detailed two-dimensional numerical study using the Finite Element Method (FEM).
000190536 6531_ $$aDielectric microparticles
000190536 6531_ $$aImmunoassay
000190536 6531_ $$aOptical detection
000190536 6531_ $$aPhotonic nanojet
000190536 700__ $$0242809$$g192120$$aYang, Hui
000190536 700__ $$aTekin, H. Cumhur
000190536 700__ $$0240260$$g110149$$aSayah, Abdeljalil
000190536 700__ $$aGijs, Martinus$$g113762$$0242801
000190536 7112_ $$dOctober 27-31, 2013$$cFreiburg, Germany$$a17th Int. Conf. on Miniaturized Systems for Chemistry and Life Sciences (microTAS '13)
000190536 773__ $$tProceedings of the 17th Int. Conf. on Miniaturized Systems for Chemistry and Life Sciences (microTAS '13)$$q1291-1293
000190536 909C0 $$xU10322$$0252094$$pLMIS2
000190536 909CO $$pconf$$pSTI$$ooai:infoscience.tind.io:190536
000190536 917Z8 $$x192120
000190536 937__ $$aEPFL-CONF-190536
000190536 973__ $$rREVIEWED$$sPUBLISHED$$aEPFL
000190536 980__ $$aCONF