000190536 001__ 190536
000190536 005__ 20180913062139.0
000190536 037__ $$aCONF
000190536 245__ $$aSelf-assembled melamine microlens arrays for immunofluorescence enhancement
000190536 269__ $$a2013
000190536 260__ $$c2013
000190536 336__ $$aConference Papers
000190536 520__ $$aSelf-assembled dielectric microlenses are used for focusing of the fluorescent signal emitted from a surface-based immunoassay, performed on silane micropatterns as assay substrates, to enhance the detection signal. In our model system, a fluorescent immunocomplex is formed on (3-aminopropyl)triethoxysilane (APTES) microstructures, and then carboxyl-functionalized melamine microparticles with two different sizes are electrostatically self-assembled on the microstructures. Mouse IgG diluted in phosphate buffered saline (PBS) is used as model target antigen and easily detected down to a concentration of 2 ng/mL. We also present a detailed two-dimensional numerical study using the Finite Element Method (FEM).
000190536 6531_ $$aDielectric microparticles
000190536 6531_ $$aImmunoassay
000190536 6531_ $$aOptical detection
000190536 6531_ $$aPhotonic nanojet
000190536 700__ $$0242809$$aYang, Hui$$g192120
000190536 700__ $$aTekin, H. Cumhur
000190536 700__ $$0240260$$aSayah, Abdeljalil$$g110149
000190536 700__ $$0242801$$aGijs, Martinus$$g113762
000190536 7112_ $$a17th Int. Conf. on Miniaturized Systems for Chemistry and Life Sciences (microTAS '13)$$cFreiburg, Germany$$dOctober 27-31, 2013
000190536 773__ $$q1291-1293$$tProceedings of the 17th Int. Conf. on Miniaturized Systems for Chemistry and Life Sciences (microTAS '13)
000190536 909C0 $$0252094$$pLMIS2$$xU10322
000190536 909CO $$ooai:infoscience.tind.io:190536$$pconf$$pSTI
000190536 917Z8 $$x192120
000190536 937__ $$aEPFL-CONF-190536
000190536 973__ $$aEPFL$$rREVIEWED$$sPUBLISHED
000190536 980__ $$aCONF