Self-assembled melamine microlens arrays for immunofluorescence enhancement

Self-assembled dielectric microlenses are used for focusing of the fluorescent signal emitted from a surface-based immunoassay, performed on silane micropatterns as assay substrates, to enhance the detection signal. In our model system, a fluorescent immunocomplex is formed on (3-aminopropyl)triethoxysilane (APTES) microstructures, and then carboxyl-functionalized melamine microparticles with two different sizes are electrostatically self-assembled on the microstructures. Mouse IgG diluted in phosphate buffered saline (PBS) is used as model target antigen and easily detected down to a concentration of 2 ng/mL. We also present a detailed two-dimensional numerical study using the Finite Element Method (FEM).


Published in:
Proceedings of the 17th Int. Conf. on Miniaturized Systems for Chemistry and Life Sciences (microTAS '13), 1291-1293
Presented at:
17th Int. Conf. on Miniaturized Systems for Chemistry and Life Sciences (microTAS '13), Freiburg, Germany, October 27-31, 2013
Year:
2013
Keywords:
Laboratories:




 Record created 2013-11-14, last modified 2018-03-17


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