Imaging cells and sub-cellular structures with ultrahigh resolution full-field X-ray microscopy

Our experimental results demonstrate that full-field hard-X-ray microscopy is finally able to investigate the internal structure of cells in tissues. This result was made possible by three main factors: the use of a coherent (synchrotron) source of X-rays, the exploitation of contrast mechanisms based on the real part of the refractive index and the magnification provided by high-resolution Fresnel zone-plate objectives. We specifically obtained high-quality microradiographs of human and mouse cells with 29 nm Rayleigh spatial resolution and verified that tomographic reconstruction could be implemented with a final resolution level suitable for subcellular features. We also demonstrated that a phase retrieval method based on a wave propagation algorithm could yield good subcellular images starting from a series of defocused microradiographs. The concluding discussion compares cellular and subcellular hard-X-ray microradiology with other techniques and evaluates its potential impact on biomedical research. (C) 2012 Elsevier Inc. All rights reserved.


Published in:
Biotechnology Advances, 31, 3, 375-386
Presented at:
European-Science-Foundation (ESF) Exploratory Workshop
Year:
2013
Publisher:
Oxford, Pergamon-Elsevier Science Ltd
ISSN:
0734-9750
Keywords:
Laboratories:




 Record created 2013-10-01, last modified 2018-01-28


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