On-the-Fly Kinetics of Enzymatic Racemization Using Deuterium NMR in DNA-Based Chiral Oriented Media

We report the in situ and real-time monitoring of the interconversion of L- and D-alanine-d(3) by alanine racemase from Bacillus stearothermophilus directly observed by H-2 NMR spectroscopy in anisotropic phase. The enantiomers are distinguished by the difference of their H-2 quadrupolar splittings in a chiral liquid crystal containing short DNA fragments. The proof-of-principle, the reliability, and the robustness of this new method is demonstrated by the determination of the turnover rates of the enzyme using the Michaelis Menten model.


Published in:
Analytical Chemistry, 85, 9, 4694-4697
Year:
2013
Publisher:
Washington, American Chemical Society
ISSN:
0003-2700
Laboratories:


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 Record created 2013-10-01, last modified 2018-09-13

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