This paper presents a novel approach for estimating the rate of turbulent kinetic energy dissipation from pulse-coherent acoustic Doppler current profiler (ADCP) measurements using the inertial dissipation method. Although the inertial dissipation technique is widely accepted and used in oceanographic and atmospheric research, its application to ADCP data is limited by the loss of directional information for high-wavenumber velocity fluctuations. However, measurements in the bottom boundary layer of a lake revealed astonishing agreement between dissipation rates estimated from temperature microstructure profiles and those estimated by applying the inertial dissipation method to data from two different brands of ADCPs.