000185541 001__ 185541
000185541 005__ 20190416220716.0
000185541 0247_ $$2doi$$a10.1093/nar/gks1260
000185541 022__ $$a0305-1048
000185541 02470 $$2ISI$$a000314121100031
000185541 037__ $$aARTICLE
000185541 245__ $$aHigh-resolution transcriptome and genome-wide dynamics of RNA polymerase and NusA in Mycobacterium tuberculosis
000185541 269__ $$a2013
000185541 260__ $$bOxford University Press$$c2013$$aOxford
000185541 300__ $$a17
000185541 336__ $$aJournal Articles
000185541 520__ $$aTo construct a regulatory map of the genome of the human pathogen, Mycobacterium tuberculosis, we applied two complementary high-resolution approaches: strand-specific RNA-seq, to survey the global transcriptome, and ChIP-seq, to monitor the genome-wide dynamics of RNA polymerase (RNAP) and the anti-terminator NusA. Although NusA does not bind directly to DNA, but rather to RNAP and/or to the nascent transcript, we demonstrate that NusA interacts with RNAP ubiquitously throughout the chromosome, and that its profile mirrors RNAP distribution in both the exponential and stationary phases of growth. Generally, promoter-proximal peaks for RNAP and NusA were observed, followed by a decrease in signal strength reflecting transcriptional polarity. Differential binding of RNAP and NusA in the two growth conditions correlated with transcriptional activity as reflected by RNA abundance. Indeed, a significant association between expression levels and the presence of NusA throughout the gene body was detected, confirming the peculiar transcription-promoting role of NusA. Integration of the data sets pinpointed transcriptional units, mapped promoters and uncovered new anti-sense and non-coding transcripts. Highly expressed transcriptional units were situated mainly on the leading strand, despite the relatively unbiased distribution of genes throughout the genome, thus helping the replicative and transcriptional complexes to align.
000185541 700__ $$0244370$$g181909$$uEcole Polytech Fed Lausanne, Global Hlth Inst, CH-1015 Lausanne, Switzerland$$aUplekar, Swapna
000185541 700__ $$uEcole Polytech Fed Lausanne, Bioinformat & Biostat Core Facil, CH-1015 Lausanne, Switzerland$$aRougemont, Jacques
000185541 700__ $$0243892$$g177247$$uEcole Polytech Fed Lausanne, Global Hlth Inst, CH-1015 Lausanne, Switzerland$$aCole, Stewart T.
000185541 700__ $$aSala, Claudia$$uEcole Polytech Fed Lausanne, Global Hlth Inst, CH-1015 Lausanne, Switzerland$$g179660$$0244366
000185541 773__ $$j41$$tNucleic Acids Research$$k2$$q961-977
000185541 8564_ $$uhttps://infoscience.epfl.ch/record/185541/files/Nucl.%20Acids%20Res.-2013-Uplekar-961-77.pdf$$zPublisher's version$$s3032409$$yPublisher's version
000185541 909C0 $$xU11742$$0252302$$pUPCOL
000185541 909CO $$ooai:infoscience.tind.io:185541$$qGLOBAL_SET$$pSV$$particle
000185541 917Z8 $$x164606
000185541 917Z8 $$x164606
000185541 917Z8 $$x182396
000185541 937__ $$aEPFL-ARTICLE-185541
000185541 973__ $$rREVIEWED$$sPUBLISHED$$aEPFL
000185541 980__ $$aARTICLE