Photonic Crystal Biosensor Based on Optical Surface Waves

A label-free biosensor device based on registration of photonic crystal surface waves is described. Angular interrogation of the optical surface wave resonance is used to detect changes in the thickness of an adsorbed layer, while an additional simultaneous detection of the critical angle of total internal reflection provides independent data of the liquid refractive index. The abilities of the device are demonstrated by measuring of biotin molecule binding to a streptavidin monolayer, and by measuring association and dissociation kinetics of immunoglobulin G proteins. Additionally, deposition of PSS / PAH polyelectrolytes is recorded in situ resulting calculation of PSS and PAH monolayer thicknesses separately.


Published in:
Sensors, 13, 2, 2566-2578
Year:
2013
Publisher:
Basel, Mdpi Ag
ISSN:
1424-8220
Keywords:
Laboratories:




 Record created 2013-03-28, last modified 2018-03-17


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