Large-Scale Production of Microcrystals and Precipitates of Proteins and Their Complexes

The optimum conditions for the formation of plate-like and urchin-like microcrystals of biomolecules and their transfer to rotors for solid-state NMR spectroscopy depend on a variety of factors, of which minimizing the manipulation of the microcrystals and storing the sample for several months at 277 K (4 degrees C) play an important role. Three biological systems were investigated: Hen Egg-White (HEW) lysozyme (129 residues), the lengthened C-terminal domain (LCter) of Human centrin 2 (89 residues), and the complex between the C-terminal domain (Cter) of Human centrin 2 (79 residues) and the P17-XPC peptide (17 residues).


Published in:
CRYSTAL GROWTH & DESIGN, 12, 12, 6199-6207
Year:
2012
Publisher:
Washington, Amer Chemical Soc
Laboratories:




 Record created 2013-03-21, last modified 2018-03-17

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