000184284 001__ 184284
000184284 005__ 20190108131327.0
000184284 0247_ $$2doi$$a10.1002/stem.1192
000184284 022__ $$a1066-5099
000184284 02470 $$2ISI$$a000308928300020
000184284 037__ $$aARTICLE
000184284 245__ $$aDual Lineage-Specific Expression of Sox17 During Mouse Embryogenesis
000184284 260__ $$bWiley-Blackwell$$c2012$$aHoboken
000184284 269__ $$a2012
000184284 300__ $$a12
000184284 336__ $$aJournal Articles
000184284 520__ $$aSox17 is essential for both endoderm development and fetal hematopoietic stem cell (HSC) maintenance. While endoderm-derived organs are well known to originate from Sox17-expressing cells, it is less certain whether fetal HSCs also originate from Sox17-expressing cells. By generating a Sox17GFPCre allele and using it to assess the fate of Sox17-expressing cells during embryogenesis, we confirmed that both endodermal and a part of definitive hematopoietic cells are derived from Sox17-positive cells. Prior to E9.5, the expression of Sox17 is restricted to the endoderm lineage. However, at E9.5 Sox17 is expressed in the endothelial cells (ECs) at the para-aortic splanchnopleural region that contribute to the formation of HSCs at a later stage. The identification of two distinct progenitor cell populations that express Sox17 at E9.5 was confirmed using fluorescence-activated cell sorting together with RNA-Seq to determine the gene expression profiles of the two cell populations. Interestingly, this analysis revealed differences in the RNA processing of the Sox17 mRNA during embryogenesis. Taken together, these results indicate that Sox17 is expressed in progenitor cells derived from two different germ layers, further demonstrating the complex expression pattern of this gene and suggesting caution when using Sox17 as a lineage-specific marker. STEM Cells2012;30:22972308
000184284 6531_ $$aEndoderm
000184284 6531_ $$aMesoderm
000184284 6531_ $$aVentral pancreas
000184284 6531_ $$aHemogenic endothelium
000184284 6531_ $$aSox17
000184284 700__ $$uVanderbilt Univ, Dept Mol Physiol & Biophys, Nashville, TN 37232 USA$$aChoi, Eunyoung
000184284 700__ $$uEcole Polytech Fed Lausanne, Swiss Inst Expt Canc Res, Sch Life Sci, Lausanne, Switzerland$$aKraus, Marine R-C.
000184284 700__ $$uEcole Polytech Fed Lausanne, Swiss Inst Expt Canc Res, Sch Life Sci, Lausanne, Switzerland$$aLemaire, Laurence A.
000184284 700__ $$uIndiana Univ Sch Med, Dept Pediat, Wells Ctr Pediat Res, Indianapolis, IN USA$$aYoshimoto, Momoko
000184284 700__ $$uIndiana Univ Sch Med, Dept Pediat, Wells Ctr Pediat Res, Indianapolis, IN USA$$aVemula, Sasidhar
000184284 700__ $$uVanderbilt Univ, Dept Mol Physiol & Biophys, Nashville, TN 37232 USA$$aPotter, Leah A.
000184284 700__ $$uUniv Penn, Sch Med, Penn Ctr Bioinformat, Philadelphia, PA 19104 USA$$aManduchi, Elisabetta
000184284 700__ $$aStoeckert, Christian J. Jr.
000184284 700__ $$0240727$$g169494$$uEcole Polytech Fed Lausanne, Swiss Inst Expt Canc Res, Sch Life Sci, Lausanne, Switzerland$$aGrapin-Botton, Anne
000184284 700__ $$uVanderbilt Univ, Sch Med, Ctr Stem Cell Biol, Nashville, TN 37232 USA$$aMagnuson, Mark A.
000184284 773__ $$j30$$tStem Cells$$k10$$q2297-2308
000184284 909C0 $$0252167$$pUPGRA$$xU11258
000184284 909CO $$particle$$ooai:infoscience.tind.io:184284
000184284 937__ $$aEPFL-ARTICLE-184284
000184284 973__ $$rREVIEWED$$sPUBLISHED$$aEPFL
000184284 980__ $$aARTICLE