000183089 001__ 183089
000183089 005__ 20190316235543.0
000183089 0247_ $$2doi$$a10.1109/JETCAS.2012.2223592
000183089 037__ $$aARTICLE
000183089 245__ $$aA Self-Contained System With CNTs-Based Biosensors for Cell Culture Monitoring
000183089 269__ $$a2012
000183089 260__ $$c2012
000183089 336__ $$aJournal Articles
000183089 520__ $$aBiosensors have been applied to disparate fields, especially for endogenous compounds such as glucose and lactate. The main areas of application are certainly related to medical and diagnostic purposes. However, metabolic monitoring can be also of interest in cell analysis. Cells can be cultivated for several purposes, such as understanding and modeling some biological mechanisms, the development of new drugs and therapies, or in the field of regenerative medicine. All the aforementioned applications require a thorough knowledge of the biological system under study. In this paper, we propose the development of a self-contained system based on electrochemical biosensors for cell culture monitoring. The detection is based on oxidases immobilized onto carbon nanotubes. We also develop an architecture to record the signal generated by the biosensor and transmit it to a remote station by means of a Bluetooth module. We calibrate the system for glucose and lactate detection in phosphate buffer solution. We achieve a sensitivity of 55.5 µA/mMcm<sup>-2</sup>and a detection limit of 2 µM for glucose, as well as a sensitivity of 25.0 µA/mMcm<sup>-2</sup> and a detection limit of 11 µM	for lactate. We finally validate the two biosensors for metabolic monitoring in culture medium and we detect lactate production in neuroblastoma cells after 72 h of cultivation. The integrated system proposed in the present work opens new opportunities towards the development of novel tools for cell analysis.
000183089 6531_ $$aamperometric biosensor
000183089 6531_ $$acarbon nanotube
000183089 6531_ $$acell culture
000183089 6531_ $$ametabolic monitoring
000183089 6531_ $$apotentiostat
000183089 700__ $$0242418$$g176461$$aBoero, Cristina
000183089 700__ $$0242419$$g183208$$aOlivo, Jacopo
000183089 700__ $$g182237$$aCarrara, Sandro$$0242413
000183089 700__ $$aDe Micheli, Giovanni$$g167918$$0240269
000183089 773__ $$j2$$tIEEE Journal on Emerging and Selected Topics in Circuits and Systems$$k4$$q658-671
000183089 8564_ $$uhttps://infoscience.epfl.ch/record/183089/files/06365265.pdf$$zn/a$$s1859160$$yn/a
000183089 909C0 $$xU11140$$0252283$$pLSI1
000183089 909CO $$particle$$ooai:infoscience.tind.io:183089$$qGLOBAL_SET$$pSTI$$pIC
000183089 917Z8 $$x112915
000183089 937__ $$aEPFL-ARTICLE-183089
000183089 973__ $$rREVIEWED$$sPUBLISHED$$aEPFL
000183089 980__ $$aARTICLE