A calcium ion-selective electrode array for monitoring the activity of HepG2/C3As in a microchannel

Variations in calcium ion (Ca2+) concentrations play a crucial role in cell-cell communication; periodic changes act as cellular signals. In this study, we developed a solid-state calcium ion-selective electrode (Ca2+ ISE) integrated in a microchannel to measure extracellular Ca2+ variations; an ISE has a high detection sensitivity (as low as 10(-9) M) and selectivity against potassium ions (K+). The sensor was used to monitor the cellular activities of HepG2/C3As cultured in a microchannel. We measured a periodically oscillating extracellular Ca2+ concentration that was superimposed on a gradual increase in the Ca2+ concentration after potassium chloride (KCl) stimulation. The oscillation period was in the range of 30-50 s, which was in good agreement with the literature.


Published in:
Sensors and Actuators B: Chemical, 174, 473-477
Year:
2012
Publisher:
Lausanne, Elsevier
ISSN:
0925-4005
Keywords:
Laboratories:


Note: The status of this file is: EPFL only


 Record created 2013-01-10, last modified 2018-03-17

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