000182422 001__ 182422
000182422 005__ 20181001181847.0
000182422 0247_ $$2doi$$a10.1021/nl3021076
000182422 022__ $$a1530-6992
000182422 02470 $$2ISI$$a000308576000048
000182422 037__ $$aARTICLE
000182422 245__ $$aQuantitative super-resolution imaging reveals protein stoichiometry and nanoscale morphology of assembling HIV-Gag virions
000182422 260__ $$aWashington$$bAmer Chemical Soc$$c2012
000182422 269__ $$a2012
000182422 300__ $$a6
000182422 336__ $$aJournal Articles
000182422 520__ $$aThe HIV structural protein Gag assembles to form spherical particles of radius ∼70 nm. During the assembly process, the number of Gag proteins increases over several orders of magnitude from a few at nucleation to thousands at completion. The challenge in studying protein assembly lies in the fact that current methods such as standard fluorescence or electron microscopy techniques cannot access all stages of the assembly process in a cellular context. Here, we demonstrate an approach using super-resolution fluorescence imaging that permits quantitative morphological and molecular counting analysis over a wide range of protein cluster sizes. We applied this technique to the analysis of hundreds of HIV-Gag clusters at the cellular plasma membrane, thus elucidating how different fluorescent labels can change the assembly of virions.
000182422 6531_ $$aBIO-IMAGING
000182422 700__ $$0245032$$aGunzenhäuser, Julia$$g166106
000182422 700__ $$0245034$$aOlivier, Nicolas$$g205242
000182422 700__ $$0245168$$aPengo, Thomas$$g209086
000182422 700__ $$0244708$$aManley, Suliana$$g189937
000182422 773__ $$j12$$k9$$q4705-10$$tNano letters
000182422 8564_ $$s1082933$$uhttps://infoscience.epfl.ch/record/182422/files/Gunzenhaeuser_GagMorpho_NanoLett2012.pdf$$yn/a$$zn/a
000182422 909C0 $$0252362$$pLEB$$xU12031
000182422 909CO $$ooai:infoscience.tind.io:182422$$pSB$$particle$$qGLOBAL_SET
000182422 917Z8 $$x173008
000182422 917Z8 $$x173008
000182422 937__ $$aEPFL-ARTICLE-182422
000182422 973__ $$aEPFL$$rREVIEWED$$sPUBLISHED
000182422 980__ $$aARTICLE