Using triplet-triplet energy transfer to measure conformational dynamics in polypeptide chains

Intrachain diffusion processes play an important role in protein folding and function. In this chapter we discuss the application of triplet-triplet energy transfer to directly measure rate constants for intrachain contact formation in polypeptide chains. The photochemistry of triplet-triplet energy transfer is described, experimental prerequisites of the method are discussed, and a detailed description of the experimental protocols and data analysis is given.


Published in:
Methods in molecular biology, 350, 169-187
Year:
2006
Keywords:
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 Record created 2012-10-15, last modified 2018-03-17


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