Proteolysis in microfluidic droplets: an approach to interface protein separation and peptide mass spectrometry

A versatile microreactor protocol based on microfluidic droplets has been developed for on-line protein digestion. Proteins separated by liquid chromatography are fractionated in water-in-oil droplets and digested in sequence. The microfluidic reactor acts also as an electrospray ionization emitter for mass spectrometry analysis of the peptides produced in the individual droplets. Each droplet is an enzymatic micro-reaction unit with efficient proteolysis due to rapid mixing, enhanced mass transfer and automated handling. This droplet approach eliminates sample loss, cross-contamination, non-specific absorption and memory effect. A protein mixture was successfully identified using the droplet-based micro-reactor as interface between reverse phase liquid chromatography and mass spectrometry.


Published in:
Lab On A Chip, 12, 2625-2629
Year:
2012
Publisher:
Royal Society of Chemistry
ISSN:
1473-0197
Keywords:
Laboratories:




 Record created 2012-07-27, last modified 2018-03-17

Publisher's version:
Download fulltextPDF
Supporting Information:
Download fulltextPDF
TOC graphic:
Download fulltextGIF
Rate this document:

Rate this document:
1
2
3
 
(Not yet reviewed)