000180159 001__ 180159
000180159 005__ 20181228151107.0
000180159 0247_ $$2doi$$a10.1117/12.922598
000180159 020__ $$a978-0-8194-9119-0
000180159 02470 $$2ISI$$a000305701200006
000180159 037__ $$aCONF
000180159 245__ $$aQuantitative Measurement of absolute cell volume and intracellular integral refractive index (RI) with Dual-wavelength Digital Holographic Microscopy (DHM)
000180159 269__ $$a2012
000180159 260__ $$bSpie-Int Soc Optical Engineering, Po Box 10, Bellingham, Wa 98227-0010 Usa$$c2012
000180159 336__ $$aConference Papers
000180159 490__ $$aProceedings of SPIE
000180159 520__ $$aQuantitative Phase Imaging techniques including DHM have been applied recently in the field of cell imaging to monitor and quantify non-invasively dynamic cellular processes modifying cell morphology and/or content. Concretely, the DHM phase signal is highly sensitive to cell thickness and intracellular integral RI variations associated with transmembrane water movements. As net water flow across the cell membrane leads at the same time to changes in cell thickness and intracellular RI, the interpretation of phase signal variations remains difficult. To overcome this drawback, we have developed a Dual-wavelength Digital Holographic Microscopy (DHM) setup allowing to separately measure, with a single CCD camera acquisition, thickness and integral RI of living cells. The method is based on the use of an absorbing dye that enhances the refractive index dispersion of the extracellular medium. Practically, two significantly different phase signals can be obtained when measuring at two appropriate wavelengths. From the two phase measurements, both cell RI and thickness can be univocally determined.
000180159 6531_ $$aDigital Holographic Microscopy
000180159 6531_ $$aAbsolute cell volume
000180159 6531_ $$aPhase decoupling procedure
000180159 6531_ $$aWater membrane permeability
000180159 6531_ $$aDry mass concentration
000180159 6531_ $$aLiving Cells
000180159 6531_ $$aMorphometry
000180159 700__ $$0243700$$g161077$$aBoss, Daniel
000180159 700__ $$aKuehn, Jonas
000180159 700__ $$0240009$$g104931$$aDepeursinge, Christian
000180159 700__ $$aMagistretti, Pierre J.$$g134990$$0243698
000180159 700__ $$aMarquet, Pierre
000180159 7112_ $$dApr 16-19, 2012$$cBrussels, BELGIUM$$aConference on Biophotonics - Photonic Solutions for Better Health Care III
000180159 773__ $$j8427$$tBiophotonics: Photonic Solutions For Better Health Care Iii$$q-
000180159 909C0 $$xU10346$$0252008$$pLOA
000180159 909C0 $$pLNDC$$xU11150$$0252265
000180159 909CO $$qSV$$pconf$$pSTI$$ooai:infoscience.tind.io:180159
000180159 917Z8 $$x182735
000180159 937__ $$aEPFL-CONF-180159
000180159 973__ $$rNON-REVIEWED$$sPUBLISHED$$aEPFL
000180159 980__ $$aCONF