000179706 001__ 179706
000179706 005__ 20190316235422.0
000179706 0247_ $$2doi$$a10.1116/1.2746873
000179706 022__ $$a1934-8630
000179706 02470 $$2ISI$$a000256466900003
000179706 037__ $$aARTICLE
000179706 245__ $$aMicrofluidic patterning of alginate hydrogels
000179706 269__ $$a2007
000179706 260__ $$bAmerican Vacuum Society$$c2007
000179706 336__ $$aJournal Articles
000179706 520__ $$aIn this article the authors present techniques which allow the microfluidic design of alginate microgels with layer composition on a chip. The hydrogel is created by combining two laminar flows of the gel precursor solutions-a calcium solution and an alginate solution-in a microchannel. The alginate solution is loaded with particles and by employing a certain fluid handling protocol involving several alginate solutions with different types of particles, a gel bar composed of many layers, each layer filled with a certain particle type, is formed. This method allows to produce defined lamellae of gel with extraordinarily small size and large aspect ratios. The minimal width attainable for a single layer by this technique is determined by the experimental conditions and for the conditions of the present article layer widths on the order of 10 mu m have been realized at a gel thickness of 100 mu m. Another method described is based on the finding that the degree of particle incorporation in the gel varies with the particle speed in the alginate flow. Altering the alginate flow rate thus allows to form a gel bar with an inner structure due to varying particle density. The authors believe that alginate gel patterning technology, which relies on easily available equipment and involves gentle particle immobilization conditions, could offer a novel approach toward the engineering of artificial tissues on the micrometer range or to cell micropatterning for analytical purposes. (C) 2007 American Vacuum Society.
000179706 6531_ $$aFabrication
000179706 6531_ $$aGel
000179706 6531_ $$aMicrostructures
000179706 6531_ $$aPh
000179706 6531_ $$aElectrophoresis
000179706 6531_ $$aImmobilization
000179706 6531_ $$aConstruction
000179706 6531_ $$aMicrogels
000179706 6531_ $$aMembranes
000179706 6531_ $$aProteins
000179706 700__ $$aJohann, Robert M.
000179706 700__ $$g107144$$aRenaud, Philippe$$0240219
000179706 773__ $$j2$$tBiointerphases$$q73-79
000179706 8564_ $$uhttps://infoscience.epfl.ch/record/179706/files/BIP000073.pdf$$zPublisher's version$$s812287$$yPublisher's version
000179706 909C0 $$xU10324$$0252064$$pLMIS4
000179706 909CO $$ooai:infoscience.tind.io:179706$$qGLOBAL_SET$$pSTI$$particle
000179706 917Z8 $$x107144
000179706 917Z8 $$x148230
000179706 937__ $$aEPFL-ARTICLE-179706
000179706 973__ $$rREVIEWED$$sPUBLISHED$$aEPFL
000179706 980__ $$aARTICLE