000178298 001__ 178298
000178298 005__ 20190416220709.0
000178298 020__ $$a978-1-4673-0219-7
000178298 0247_ $$2doi$$a10.1109/ISCAS.2012.6271688
000178298 037__ $$aCONF
000178298 245__ $$aQuantitative Comparison of Commercial CCD and Custom-Designed CMOS Camera for Biological Applications
000178298 269__ $$a2012
000178298 260__ $$c2012
000178298 336__ $$aConference Papers
000178298 520__ $$aIn biological applications and systems where even the smallest details have a meaning, CCD cameras are mostly preferred and they hold most of the market share despite their high costs. In this paper, we propose a custom-designed CMOS camera to compete with the default CCD camera of an inverted microscope for fluorescence imaging. The custom-designed camera includes a commercially available mid-performance CMOS image sensor and a Field-Programmable Gate Array (FPGA) based hardware platform (FPGA4U). The high cost CCD camera of the microscope is replaced by the custom-designed CMOS camera and the two are quantitatively compared for a specific application where an Estrogen Reception (ER) expression in breast cancer diagnostic samples that emits light at 665nm has been imaged by both cameras. The gray-scale images collected by both cameras show a very similar intensity distribution. In addition, normalized white pixels after thresholding resulted in 4.96% for CCD and 3.38% for CMOS. The results and images after thresholding show that depending on the application even a mid-performance CMOS camera can provide enough image quality when the target is localization of fluorescent stained biological details. Therefore the cost of the cameras can be drastically reduced while benefiting from the inherent advantages of CMOS devices plus adding more features and flexibility to the camera systems with FPGAs.
000178298 6531_ $$aCMOS camera
000178298 6531_ $$aCCD camera
000178298 6531_ $$abiological applications
000178298 6531_ $$atissue samples
000178298 6531_ $$afluorescence imaging
000178298 6531_ $$alow light imaging
000178298 6531_ $$ahigh cost cameras
000178298 6531_ $$aFPGA camera
000178298 6531_ $$aCMOS image sensor
000178298 700__ $$0242423$$g175952$$aKöklü, Gözen
000178298 700__ $$0245236$$g180014$$aGhaye, Julien Michel
000178298 700__ $$0243374$$g100366$$aBeuchat, René
000178298 700__ $$0240269$$g167918$$aDe Micheli, Giovanni
000178298 700__ $$g112194$$aLeblebici, Yusuf$$0240162
000178298 700__ $$0242413$$g182237$$aCarrara, Sandro
000178298 7112_ $$dMay 20-23, 2012$$cSeoul, Korea$$a2012 IEEE International Symposium on Circuits and Systems (ISCAS 2012)
000178298 773__ $$tProceedings of 2012 IEEE International Symposium on Circuits and Systems$$q2063-2066
000178298 8564_ $$uhttp://ieeexplore.ieee.org/xpls/abs_all.jsp?arnumber=6271688$$zURL
000178298 8564_ $$uhttps://infoscience.epfl.ch/record/178298/files/2063.pdf$$zPublisher's version$$s634578$$yPublisher's version
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000178298 909CO $$qGLOBAL_SET$$pconf$$pSTI$$pIC$$ooai:infoscience.tind.io:178298
000178298 917Z8 $$x175952
000178298 917Z8 $$x175952
000178298 917Z8 $$x175952
000178298 937__ $$aEPFL-CONF-178298
000178298 973__ $$rREVIEWED$$sPUBLISHED$$aEPFL
000178298 980__ $$aCONF