000177927 001__ 177927
000177927 005__ 20180913061321.0
000177927 0247_ $$2doi$$a10.1016/j.humimm.2012.02.019
000177927 022__ $$a0198-8859
000177927 02470 $$2ISI$$a000304024500004
000177927 037__ $$aARTICLE
000177927 245__ $$aDifferential peptide binding to CD40 evokes counteractive responses
000177927 260__ $$bElsevier$$c2012
000177927 269__ $$a2012
000177927 336__ $$aJournal Articles
000177927 520__ $$aThe antigen-presenting cell-expressed CD40 is implied in the regulation of counteractive immune responses such as induction of pro-inflammatory and anti-inflammatory cytokines interleukin (IL)-12 and IL-10, respectively. The mechanism of this duality in CD40 function remains unknown. Here, we investigated whether such duality depends on ligand binding. Based on CD40 binding, we identifed two dodecameric peptides, peptide-7 and peptide-19, from the phage peptide library. Peptide-7 induces IL-10 and increases Leishmania donovani infection in macrophages, whereas peptide-19 induces IL-12 and reduces L donovani infection. CD40-peptide interaction analyses by surface plasmon resonance and atomic force microscopy suggest that the functional differences are not associated with the studied interaction parameters. The molecular dynamic simulation of the CD40-peptides interaction suggests that these two peptides bind to two different places on CD40. Thus, we suggest for the first time that differential binding of the ligands imparts functional duality to CD40. (C) 2012 American Society for Histocompatibility and Immunogenetics. Published by Elsevier Inc. All rights reserved.
000177927 6531_ $$aLeishmania
000177927 6531_ $$aMacrophages
000177927 6531_ $$aCd40
000177927 6531_ $$aCD40-ligand
000177927 6531_ $$aCostimulation
000177927 6531_ $$aReceptor
000177927 6531_ $$aLigand
000177927 700__ $$aKhan, Srijit
000177927 700__ $$0243240$$aAlonso, Livan$$g182967
000177927 700__ $$0240089$$aRoduit, Charles$$g103412
000177927 700__ $$aBandyopadhyay, Syamdas
000177927 700__ $$aSingh, Shailza
000177927 700__ $$aSaha, Shipra
000177927 700__ $$aTacchini-Cottier, Fabienne
000177927 700__ $$aRoy, Somenath
000177927 700__ $$0240091$$aDietler, Giovanni$$g125942
000177927 700__ $$0240307$$aKasas, Sandor$$g150334
000177927 700__ $$aDas, Pradeep
000177927 700__ $$aKrishnasastry, M. V.
000177927 700__ $$aSaha, Bhaskar
000177927 773__ $$j73$$q465-469$$tHuman Immunology
000177927 909C0 $$0252162$$pLPMV$$xU10868
000177927 909CO $$ooai:infoscience.tind.io:177927$$pSB$$particle
000177927 917Z8 $$x125942
000177927 937__ $$aEPFL-ARTICLE-177927
000177927 973__ $$aEPFL$$rREVIEWED$$sPUBLISHED
000177927 980__ $$aARTICLE