000177534 001__ 177534
000177534 005__ 20181203022729.0
000177534 0247_ $$2doi$$a10.1097/00004647-200211000-00008
000177534 022__ $$a0271-678X
000177534 037__ $$aARTICLE
000177534 245__ $$aEffect of deep pentobarbital anesthesia on neurotransmitter metabolism in vivo: on the correlation of total glucose consumption with glutamatergic action
000177534 269__ $$a2002
000177534 260__ $$bNature Publishing Group$$c2002
000177534 336__ $$aJournal Articles
000177534 520__ $$aThe effect of deep barbiturate anesthesia on brain glucose transport, TCA cycle flux, and aspartate, glutamate, and glutamine metabolism was assessed in the rat brain using 13C nuclear magnetic resonance spectroscopy at 9.4 T in conjunction with [1-13C] glucose infusions. Brain glucose concentrations were elevated, consistent with a twofold reduced cerebral metabolic rate for glucose (CMRglc) compared with light alpha-chloralose anesthesia. Using a mathematical model of neurotransmitter metabolism, several metabolic reaction rates were extracted from the rate of label incorporation. Total oxidative glucose metabolism, CMRglc(ox), was 0.33 +/- 0.03 micromol x g(-1) x min(-1). The neuronal TCA cycle rate was similar to that in the glia, 0.35 +/- 0.03 micromol x g(-1) x min(-1) and 0.26 +/- 0.06 micromol x g(-1) x min(-1), respectively, suggesting that neuronal energy metabolism was mainly affected. The rate of pyruvate carboxylation was 0.03 +/- 0.01 micromol x g(-1) x min(-1). The exchange rate between cytosolic glutamate and mitochondrial 2-oxoglutarate, Vx, was equal to the rate of neuronal pyruvate dehydrogenase flux. This indicates that Vx is coupled to CMRglc(ox), implying that the malate-aspartate shuttle is the major mechanism that facilitates label exchange across the inner mitochondrial membrane. The apparent rate of glutamatergic neurotransmission, V(NT), was 0.04 +/- 0.01 micromol x g x min, consistent with strong reductions in electrical activity. However, the rates of cerebral oxidative glucose metabolism and glutamatergic neurotransmission, CMRglc(ox)/V(NT), did not correlate with a 1:1 stoichiometry.
000177534 6531_ $$aAnesthesia, General
000177534 6531_ $$aPentobarbital
000177534 700__ $$aChoi, In-Young
000177534 700__ $$0243741$$aLei, Hongxia$$g170746
000177534 700__ $$0243712$$aGruetter, Rolf$$g161735
000177534 773__ $$j22$$k11$$q1343-51$$tJournal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism
000177534 8564_ $$s161380$$uhttps://infoscience.epfl.ch/record/177534/files/Choi_PB_2002.pdf$$yPublisher's version$$zPublisher's version
000177534 909C0 $$0252276$$pLIFMET$$xU10984
000177534 909CO $$ooai:infoscience.tind.io:177534$$pSB$$particle
000177534 917Z8 $$x161735
000177534 937__ $$aEPFL-ARTICLE-177534
000177534 973__ $$aOTHER$$rREVIEWED$$sPUBLISHED
000177534 980__ $$aARTICLE