000177320 001__ 177320
000177320 005__ 20190316235356.0
000177320 0247_ $$2doi$$a10.3390/s120506520
000177320 02470 $$2ISI$$a000304539200077
000177320 037__ $$aARTICLE
000177320 041__ $$aeng
000177320 245__ $$aElectrochemical Detection of Anti-Breast-Cancer Agents in Human Serum by Cytochrome P450-Coated Carbon Nanotubes
000177320 269__ $$a2012
000177320 260__ $$c2012
000177320 336__ $$aJournal Articles
000177320 520__ $$aWe report on the electrochemical detection of anti-cancer drugs in human serum with sensitivity values in the range of 8–925 nA/µM. Multi-walled carbon nanotubes were functionalized with three different cytochrome P450 isoforms (CYP1A2, CYP2B6, and CYP3A4). A model used to effectively describe the cytochrome P450 deposition onto carbon nanotubes was confirmed by Monte Carlo simulations. Voltammetric measurements were performed in phosphate buffer saline (PBS) as well as in human serum, giving well-defined current responses upon addition of increasing concentrations of anti-cancer drugs. The results assert the capability to measure concentration of drugs in the pharmacological ranges in human serum. Another important result is the possibility to detect pairs of drugs present in the same sample, which is highly required in case of therapies with high side-effects risk and in anti-cancer pharmacological treatments based on mixtures of different drugs. Our technology holds potentials for inexpensive multi-panel drug-monitoring in personalized therapy.
000177320 6531_ $$acytochrome P450
000177320 6531_ $$acarbon-nanotubes
000177320 6531_ $$aelectrochemical drugs detection
000177320 6531_ $$abreast-cancer
000177320 6531_ $$ahuman serum
000177320 6531_ $$apersonalized therapy
000177320 700__ $$0245318$$g198938$$aBaj-Rossi, Camilla
000177320 700__ $$0240269$$g167918$$aDe Micheli, Giovanni
000177320 700__ $$0242413$$g182237$$aCarrara, Sandro
000177320 773__ $$j12$$tSensors$$k5$$q6520-6537
000177320 8564_ $$uhttps://infoscience.epfl.ch/record/177320/files/sensors-12-06520.pdf$$zn/a$$s675383$$yn/a
000177320 909C0 $$xU11140$$0252283$$pLSI1
000177320 909CO $$particle$$qGLOBAL_SET$$ooai:infoscience.tind.io:177320$$pSTI$$pIC
000177320 917Z8 $$x112915
000177320 937__ $$aEPFL-ARTICLE-177320
000177320 973__ $$rREVIEWED$$sPUBLISHED$$aEPFL
000177320 980__ $$aARTICLE