Comparison of Three Methods of Biocompatible Multi-Walled Carbon Nanotubes Confinement for the Development of Implantable Amperometric Adenosine-5′-Triphosphate Biosensors

An amperometric biosensor for adenosine 5 -triphosphate (ATP) was developed applying a competitive assay of two glucose converting enzymes: glucose oxidase (GOD) and hexokinase (HEX). Competition between GOD and HEX for glucose in presence of ATP, lead to a decrease in the current coming from the hydrogen peroxide generated by the GOD, and allows ATP detection. The biosensor was realized on commercial screen-printed electrodes modified with carbon nanotubes (CNTs). Confinement of CNTs in a polycarbonate membrane, Chitosan and Nafion polymers was investigated as possible solutions for implantable sensors. Nafion gave the best performances, with a sensitivity of 25 pA/μM mm-2, and a detection limit of 257 M. The sensor resulted able to measure ATP concentrations in the range of hundred of μmol/l.


Published in:
Sensor Letters, 9, 5, 1838-1844
Year:
2011
Publisher:
American Scientific Publishers
ISSN:
1546-198X
Keywords:
Laboratories:


Note: The status of this file is: EPFL only


 Record created 2012-05-07, last modified 2018-03-17

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