Continuous and label-free toxicity screening of human hepatocytes on chip reveals frequency-dependant impedance profiles

We present an electrical impedance based biosensor on a microfluidic cell culture platform that enables label-free and re- al-time investigation of drug toxicity-induced effects on hepatocytes. The temporal evolution of the impedance at different frequencies strongly suggests distinct information on toxicity effects within the cell culture. The findings indicate that the sen- sor distinguishes between morphological changes and severe cell injury within a cell population owing to the combination of high-density culture, perfusion flow and whole impedance-frequency spectrum analysis. More importantly, cellular changes can be sensed long before cellular death, offering a highly sensitive technique over standard viability assays.


Published in:
Proceedings of the 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2011), 1487-1489
Presented at:
15th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2011), Seattle, Washington, USA, October 2-6, 2011
Year:
2011
ISBN:
978-0-9798064-4-5
Keywords:
Laboratories:


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 Record created 2012-04-30, last modified 2018-03-17

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