000175856 001__ 175856
000175856 005__ 20181203022649.0
000175856 0247_ $$2doi$$a10.1128/MCB.06318-11
000175856 022__ $$a0270-7306
000175856 02470 $$2ISI$$a000300628500013
000175856 037__ $$aARTICLE
000175856 245__ $$aRNA Profiling and Chromatin Immunoprecipitation-Sequencing Reveal that PTF1a Stabilizes Pancreas Progenitor Identity via the Control of MNX1/HLXB9 and a Network of Other Transcription Factors
000175856 260__ $$bAmerican Society for Microbiology$$c2012
000175856 269__ $$a2012
000175856 336__ $$aJournal Articles
000175856 520__ $$aPancreas development is initiated by the specification and expansion of a small group of endodermal cells. Several transcription factors are crucial for progenitor maintenance and expansion, but their interactions and the downstream targets mediating their activity are poorly understood. Among those factors, PTF1a, a basic helix-loop-helix (bHLH) transcription factor which controls pancreas exocrine cell differentiation, maintenance, and functionality, is also needed for the early specification of pancreas progenitors. We used RNA profiling and chromatin immunoprecipitation (ChIP) sequencing to identify a set of targets in pancreas progenitors. We demonstrate that Mnx1, a gene that is absolutely required in pancreas progenitors, is a major direct target of PTF1a and is regulated by a distant enhancer element. Pdx1, Nkx6.1, and Onecut1 are also direct PTF1a targets whose expression is promoted by PTF1a. These proteins, most of which were previously shown to be necessary for pancreas bud maintenance or formation, form a transcription factor network that allows the maintenance of pancreas progenitors. In addition, we identify Bmp7, Nr5a2, RhoV, and P2rx1 as new targets of PTF1a in pancreas progenitors.
000175856 6531_ $$aHuman Growth-Hormone
000175856 6531_ $$aGene-Expression
000175856 6531_ $$aDorsal Pancreas
000175856 6531_ $$aTransgenic Mice
000175856 6531_ $$aAcinar-Cells
000175856 6531_ $$aArea-Iii
000175856 6531_ $$aRbp-J
000175856 6531_ $$aPdx1
000175856 6531_ $$aExocrine
000175856 6531_ $$aDifferentiation
000175856 700__ $$aThompson, Nancy
000175856 700__ $$0243263$$aGesina, Emilie$$g177023
000175856 700__ $$aScheinert, Peter
000175856 700__ $$0244404$$aBucher, Philipp$$g113607
000175856 700__ $$0240727$$aGrapin-Botton, Anne$$g169494
000175856 773__ $$j32$$k6$$q1189-1199$$tMolecular And Cellular Biology
000175856 909C0 $$0252244$$pGR-BUCHER$$xU11780
000175856 909C0 $$0252167$$pUPGRA$$xU11258
000175856 909CO $$ooai:infoscience.tind.io:175856$$pSV$$particle
000175856 917Z8 $$x182396
000175856 937__ $$aEPFL-ARTICLE-175856
000175856 973__ $$aEPFL$$rREVIEWED$$sPUBLISHED
000175856 980__ $$aARTICLE