000175756 001__ 175756
000175756 005__ 20181203022647.0
000175756 0247_ $$2doi$$a10.1242/dev.076794
000175756 02470 $$2ISI$$a000300402400009
000175756 037__ $$aARTICLE
000175756 245__ $$aBimodal control of Hoxd gene transcription in the spinal cord defines two regulatory subclusters
000175756 269__ $$a2012
000175756 260__ $$c2012
000175756 336__ $$aJournal Articles
000175756 520__ $$aThe importance of Hox genes in the specification of neuronal fates in the spinal cord has long been recognized. However, the transcriptional controls underlying their collinear expression domains remain largely unknown. Here we show in mice that the correspondence between the physical order of Hoxd genes and their rostral expression boundaries, although respecting spatial collinearity, does not display a fully progressive distribution. Instead, two major anteroposterior boundaries are detected, coinciding with the functional subdivision of the spinal cord. Tiling array analyses reveal two distinct blocks of transcription, regulated independently from one another, that define the observed expression boundaries. Targeted deletions in vivo that remove the genomic fragments separating the two blocks induce ectopic expression of posterior genes. We further evaluate the independent regulatory potential and transcription profile of each gene locus by a tiling array approach using a contiguous series of transgenes combined with locus-specific deletions. Our work uncovers a bimodal type of HoxD spatial collinearity in the developing spinal cord that relies on two separate 'enhancer mini-hubs' to ensure correct Hoxd gene expression levels while maintaining their appropriate anteroposterior boundaries.
000175756 6531_ $$aHox genes
000175756 6531_ $$aCollinearity
000175756 6531_ $$aTranscriptional regulation
000175756 6531_ $$aMouse genetics
000175756 6531_ $$aMotor-Neuron Diversity
000175756 6531_ $$aHomeobox Gene
000175756 6531_ $$aTargeted Disruption
000175756 6531_ $$aEarly Vertebrates
000175756 6531_ $$aSubtype Identity
000175756 6531_ $$aNervous-System
000175756 6531_ $$aIn-Vivo
000175756 6531_ $$aExpression
000175756 6531_ $$aMouse
000175756 6531_ $$aOrganization
000175756 700__ $$aTschopp, Patrick
000175756 700__ $$aChristen, Alix J.
000175756 700__ $$g141236$$aDuboule, Denis$$0240276
000175756 773__ $$j139$$tDevelopment$$q929-939
000175756 909C0 $$xU11705$$0252099$$pUPDUB
000175756 909CO $$pSV$$particle$$ooai:infoscience.tind.io:175756
000175756 917Z8 $$x182482
000175756 937__ $$aEPFL-ARTICLE-175756
000175756 973__ $$rREVIEWED$$sPUBLISHED$$aEPFL
000175756 980__ $$aARTICLE