Evaluation of uptake and transport of ultrasmall superparamagnetic iron oxide nanoparticles by human brain-derived endothelial cells
Aim: Ultrasmall superparamagnetic iron oxide nanoparticles (USPIO-NPs) are under development for imaging and drug delivery; however, their interaction with human blood brain barrier models is not known. Materials & Methods: The uptake, reactive oxygen species production and transport of USPIO-NPs across human brain-derived endothelial cells as models of the blood brain tumor barrier were evaluated for either uncoated, oleic acid-coated or polyvinylamine-coated USPIO-NPs. Results: Reactive oxygen species production was observed for oleic acid-coated and polyvinylamine-coated USPIO-NPs. The uptake and intracellular localization of the iron oxide core of the USPIO-NPs was confirmed by transmission electron microscopy. However, while the uptake of these USPIO-NPs by cells was observed, they were neither released by nor transported across these cells even in the presence of an external dynamic magnetic field. Conclusion: USPIO-NP-loaded filopodia were observed to invade the polyester membrane, suggesting that they can be transported by migrating angiogenic brain-derived endothelial cells.
Keywords: cell uptake ; electron microscopy ; human brain-derived endothelial cells ; iron oxide nanoparticles ; magnetic enhancement ; oxidative stress ; transcellular transport ; Poly(Butyl Cyanoacrylate) Nanoparticles ; Drug-Delivery ; Plga Nanoparticles ; Oxidative Stress ; In-Vitro ; Barrier ; Doxorubicin ; Toxicity ; Glioblastoma ; Permeability
Record created on 2012-03-15, modified on 2016-08-09