Triplet imaging of oxygen consumption during the contraction of a single smooth muscle cell (A7r5)

Triplet imaging is a novel optical technique that allows investigating oxy- gen metabolism at the single cell and the sub-cellular level. The method combines high temporal and spatial resolution, which are required for the monitoring of fast kinetics of oxygen concentration in living cells. Calibration and validation is demon- strated with a titration experiment using L-Ascorbic Acid with the enzyme Ascor- base oxidase. The method was applied to a biological cell system, employing as reporter a cytosolic fusion protein of β-galactosidase with SNAP-tag labeled with tetramethylrhodamine. Oxygen consumption in single smooth muscle cells A7r5 during an [Arg8]-vasopressin-induced contraction is measured. The results indicate a consumption leading to an intracellular oxygen concentration that decays mono- exponentially with time. This is in good agreement with previously reported mea- surements of oxygen consumption in skeletal muscle fibers.


Published in:
Oxygen Transport to Tissue XXXIII, 263-268
Presented at:
Conference of international society on oxygen transport to tissue, Monte Verità, Ascona, Switzerland, July 18-23, 2010
Year:
2012
Publisher:
Berlin, Springer Science+Business Media
ISBN:
9781461415657
Laboratories:


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 Record created 2012-02-24, last modified 2018-10-01

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