000174610 001__ 174610
000174610 005__ 20181203022624.0
000174610 022__ $$a1098-5530
000174610 02470 $$2ISI$$a000299966000014
000174610 0247_ $$2doi$$a10.1128/JB.06417-11
000174610 037__ $$aARTICLE
000174610 245__ $$aEspD Is Critical for the Virulence-Mediating ESX-1 Secretion System in Mycobacterium tuberculosis
000174610 269__ $$a2011
000174610 260__ $$c2011
000174610 336__ $$aJournal Articles
000174610 520__ $$aESAT-6 system 1 (ESX-1)-mediated secretion in Mycobacterium tuberculosis is dependent on proteins encoded by the cotranscribed espA-espC-espD gene cluster. While the roles of EspA and EspC with respect to the ESX-1 secretion system have been actively investigated, the function of EspD remains unknown. We show that EspD is secreted by M. tuberculosis, but unlike EspA and EsxA, its export does not exclusively require the ESX-1 system. Evidence for stabilization of cellular levels of EspA and EspC by EspD is presented, and depletion of EspD results in loss of EsxA secretion. Site-directed mutagenesis of EspD reveals that its role in the maintenance of cellular levels of EspA in M. tuberculosis is distinct from its facilitation of EsxA secretion. The same mutagenesis experiments have also shown that secretion of EspD is not required for the secretion of EsxA. Our findings highlight a critical and complex role for EspD in modulating the ESX-1 secretion system in M. tuberculosis.
000174610 6531_ $$aComplex-Formation
000174610 6531_ $$aEsat-6 Secretion
000174610 6531_ $$aCalmette-Guerin
000174610 6531_ $$aAttenuation
000174610 6531_ $$aGenes
000174610 6531_ $$aRd1
000174610 6531_ $$aImmunogenicity
000174610 6531_ $$aTransformation
000174610 6531_ $$aTranscription
000174610 6531_ $$aInfection
000174610 700__ $$aChen, Jeffrey M.
000174610 700__ $$aBoy-Röttger, Stefanie
000174610 700__ $$0244375$$g178252$$aDhar, Neeraj
000174610 700__ $$aSweeney, Nathan
000174610 700__ $$aBuxton, Roger S.
000174610 700__ $$0244369$$g180776$$aPojer, Florence
000174610 700__ $$aRosenkrands, Ida
000174610 700__ $$aCole, Stewart T.$$g177247$$0243892
000174610 773__ $$j194$$tJournal of bacteriology$$k4$$q884-93
000174610 909C0 $$xU11741$$0252303$$pUPKIN
000174610 909C0 $$xU11742$$0252302$$pUPCOL
000174610 909CO $$pSV$$particle$$ooai:infoscience.tind.io:174610
000174610 917Z8 $$x164606
000174610 917Z8 $$x164606
000174610 937__ $$aEPFL-ARTICLE-174610
000174610 973__ $$rREVIEWED$$sPUBLISHED$$aEPFL
000174610 980__ $$aARTICLE