ZFN-Site searches genomes for zinc finger nuclease target sites and off-target sites

Background: Zinc Finger Nucleases (ZFNs) are man-made restriction enzymes useful for manipulating genomes by cleaving target DNA sequences. ZFNs allow therapeutic gene correction or creation of genetically modified model organisms. ZFN specificity is not absolute; therefore, it is essential to select ZFN target sites without similar genomic off-target sites. It is important to assay for off-target cleavage events at sites similar to the target sequence.


Publié dans:
BMC Bioinformatics, 12, 152
Année
2011
Publisher:
BioMed Central
ISSN:
1471-2105
Mots-clefs:
Laboratoires:




 Notice créée le 2011-12-16, modifiée le 2019-03-16

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