000171305 001__ 171305
000171305 005__ 20190316235239.0
000171305 0247_ $$2doi$$a10.1186/1471-2105-12-152
000171305 022__ $$a1471-2105
000171305 02470 $$2ISI$$a000291659600002
000171305 037__ $$aARTICLE
000171305 245__ $$aZFN-Site searches genomes for zinc finger nuclease target sites and off-target sites
000171305 269__ $$a2011
000171305 260__ $$bBioMed Central$$c2011
000171305 336__ $$aJournal Articles
000171305 520__ $$aBackground: Zinc Finger Nucleases (ZFNs) are man-made restriction enzymes useful for manipulating genomes by cleaving target DNA sequences. ZFNs allow therapeutic gene correction or creation of genetically modified model organisms. ZFN specificity is not absolute; therefore, it is essential to select ZFN target sites without similar genomic off-target sites. It is important to assay for off-target cleavage events at sites similar to the target sequence.
000171305 6531_ $$aArtificial Transcription Factors
000171305 6531_ $$aDouble-Strand Breaks
000171305 6531_ $$aDna-Sequences
000171305 6531_ $$aMajor Determinant
000171305 6531_ $$aDomains
000171305 6531_ $$aRecognition
000171305 6531_ $$aFamily
000171305 6531_ $$aCells
000171305 6531_ $$aConstruction
000171305 6531_ $$aDimerization
000171305 700__ $$aCradick, Thomas J.
000171305 700__ $$0243605$$g176602$$aAmbrosini, Giovanna
000171305 700__ $$aIseli, Christian
000171305 700__ $$g113607$$aBucher, Philipp$$0244404
000171305 700__ $$aMcCaffrey, Anton P.
000171305 773__ $$j12$$tBMC Bioinformatics$$q152
000171305 8564_ $$uhttps://infoscience.epfl.ch/record/171305/files/1471-2105-12-152.pdf$$zPublisher's version$$s3604995$$yPublisher's version
000171305 909C0 $$xU11780$$0252244$$pGR-BUCHER
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000171305 937__ $$aEPFL-ARTICLE-171305
000171305 973__ $$rREVIEWED$$sPUBLISHED$$aEPFL
000171305 980__ $$aARTICLE