ZFN-Site searches genomes for zinc finger nuclease target sites and off-target sites

Background: Zinc Finger Nucleases (ZFNs) are man-made restriction enzymes useful for manipulating genomes by cleaving target DNA sequences. ZFNs allow therapeutic gene correction or creation of genetically modified model organisms. ZFN specificity is not absolute; therefore, it is essential to select ZFN target sites without similar genomic off-target sites. It is important to assay for off-target cleavage events at sites similar to the target sequence.


Published in:
BMC Bioinformatics, 12, 152
Year:
2011
Publisher:
BioMed Central
ISSN:
1471-2105
Keywords:
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 Record created 2011-12-16, last modified 2018-03-17

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