000170385 001__ 170385
000170385 005__ 20180913060904.0
000170385 0247_ $$2doi$$a10.1371/journal.pone.0026187
000170385 022__ $$a1932-6203
000170385 02470 $$2ISI$$a000295978600043
000170385 037__ $$aARTICLE
000170385 245__ $$aSynergistic NGF/B27 Gradients Position Synapses Heterogeneously in 3D Micropatterned Neural Cultures
000170385 269__ $$a2011
000170385 260__ $$bPublic Library of Science$$c2011
000170385 336__ $$aJournal Articles
000170385 520__ $$aNative functional brain circuits show different numbers of synapses (synaptic densities) in the cerebral cortex. Until now, different synaptic densities could not be studied in vitro using current cell culture methods for primary neurons. Herein, we present a novel microfluidic based cell culture method that combines 3D micropatterning of hydrogel layers with linear chemical gradient formation. Micropatterned hydrogels were used to encapsulate dissociated cortical neurons in laminar cell layers and neurotrophic factors NGF and B27 were added to influence the formation of synapses. Neurotrophic gradients allowed for the positioning of distinguishable synaptic densities throughout a 3D micropatterned neural culture. NGF and B27 gradients were maintained in the microfluidic device for over two weeks without perfusion pumps by utilizing a refilling procedure. Spatial distribution of synapses was examined with a pre-synaptic marker to determine synaptic densities. From our experiments, we observed that (1) cortical neurons responded only to synergistic NGF/B27 gradients, (2) synaptic density increased proportionally to synergistic NGF/B27 gradients; (3) homogeneous distribution of B27 disturbed cortical neurons in sensing NGF gradients and (4) the cell layer position significantly impacted spatial distribution of synapses.
000170385 6531_ $$aNerve Growth-Factor
000170385 6531_ $$aImmobilized Concentration Gradients
000170385 6531_ $$aGuide Neurite Outgrowth
000170385 6531_ $$aAdult Sensory Neurons
000170385 6531_ $$aCortical Thickness
000170385 6531_ $$aFactor-I
000170385 6531_ $$aSomatosensory Cortex
000170385 6531_ $$aHippocampal-Neurons
000170385 6531_ $$aAlzheimers-Disease
000170385 6531_ $$aInsulin
000170385 700__ $$0242554$$aKunze, Anja$$g168829
000170385 700__ $$0242543$$aValero, Ana$$g178347
000170385 700__ $$0242934$$aZosso, Dominique$$g148664
000170385 700__ $$0240219$$aRenaud, Philippe$$g107144
000170385 773__ $$j6$$k10$$qe26187.1-12$$tPLoS ONE
000170385 8564_ $$s2108299$$uhttps://infoscience.epfl.ch/record/170385/files/journal.pone.0026187.pdf$$yPublisher's version$$zPublisher's version
000170385 909C0 $$0252394$$pLTS5$$xU10954
000170385 909C0 $$0252064$$pLMIS4$$xU10324
000170385 909CO $$ooai:infoscience.tind.io:170385$$pSTI$$particle
000170385 917Z8 $$x113143
000170385 917Z8 $$x148230
000170385 937__ $$aEPFL-ARTICLE-170385
000170385 973__ $$aEPFL$$rREVIEWED$$sPUBLISHED
000170385 980__ $$aARTICLE