Reconstructed ancestral sequences improve pathogen identification using resequencing DNA microarrays

We describe the benefit of using reconstructed ancestral sequences (RAS) on resequencing microarrays for rapid pathogen identification, with Enterobacteriaceae rpoB sequences as a model. Our results demonstrate a sharp improvement of call rate and accuracy when using RASs as compared to extant sequences. This improvement was attributed to the lower sequence divergence of RASs, which also expanded the sequence space covered by the microarray. Extension of this novel microarray design strategy to viruses, antimicrobial resistance elements or toxins is straightforward.


Published in:
PloS one, 5, 12, e15243
Year:
2010
Publisher:
Public Library of Science
ISSN:
1932-6203
Keywords:
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 Record created 2011-11-24, last modified 2018-03-17

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