000168750 001__ 168750
000168750 005__ 20190522142435.0
000168750 0247_ $$2doi$$a10.1016/j.femsec.2004.02.004
000168750 022__ $$a0168-6496
000168750 037__ $$aARTICLE
000168750 245__ $$aImpacts of heavy metal contamination and phytoremediation on a microbial community during a twelve-month microcosm experiment
000168750 260__ $$c2004$$bBlackwell Publishing Ltd, Oxford
000168750 269__ $$a2004
000168750 336__ $$aJournal Articles
000168750 500__ $$aNational Licences
000168750 520__ $$aThe effects of heavy metals and phytoextraction practices on a soil microbial Community were studied during 12 months using a hyperaccumulating plant (Thlaspi caerulcseens) grown in an artificially contaminated soil. The 16S ribosomal RNA genes of the Bacteria and the beta-Proteobacteria and the amoA gene (encoding the a-subunit of ammonia monooxygenase) were PCR-amplified and analysed by denaturing gradient gel electrophoresis (DGGE). Principal component analysis (PCA) of the DGGE data revealed that: (i) the heavy metals had the most drastic effects on the bacterial groups targeted, (ii) the plant induced changes which could be observed in the amoA and in the Bacteria 16S rRNA gene patterns, (iii) the changes observed during 12 months in the DGGE-patterns of the planted contaminated soil did not indicate recovery of the initial bacterial community present in the non-contaminated soil. The potential function of the microbial community was assessed recording community level physiological profiles (CLPP) and analysing them by PCA. The lower capability of the bacterial community to degrade the substrates provided in the BIOLOG plates, in particular the amino acids, amides and amines, as well as a delay in the average well colour development (AWCD) differentiated the bacterial community of the contaminated samples from that of the non-contaminated ones. However, the plant had a positive effect on substrate utilization as shown by the greater number of substrates used in all planted samples compared to implanted ones. Finally, the measurement of the potential ammonia oxidation indicated that ammonia oxidising bacteria were completely inhibited in the contaminated soil. The stimulation of ammonia oxidation by the plant observed in the non-contaminated samples was surpassed by the inhibitory effect of the heavy metals in the contaminated soil. This study emphasises the combined use of culture-independent techniques with conventional methods to investigate the ecology of bacteria in their natural habitats. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
000168750 6531_ $$aheavy metal contaminated soil
000168750 6531_ $$aDgge
000168750 6531_ $$aClpp
000168750 6531_ $$apotential ammonia-oxidation
000168750 6531_ $$aThlaspi caerulescens
000168750 6531_ $$arhizosphere
000168750 6531_ $$aAmmonia-Oxidizing Bacteria
000168750 6531_ $$aGradient Gel-Electrophoresis
000168750 6531_ $$aCarbon-Source Utilization
000168750 6531_ $$aHyperaccumulator Thlaspi-Caerulescens
000168750 6531_ $$aSubstrate Utilization Patterns
000168750 6531_ $$aArbuscular Mycorrhizal Fungi
000168750 6531_ $$a16S Ribosomal-Rna
000168750 6531_ $$aCatabolic Versatility
000168750 6531_ $$aStatistical-Analysis
000168750 6531_ $$aManagement Regimens
000168750 700__ $$aChatzinotas, A.
000168750 700__ $$aKaufmann, K.
000168750 700__ $$aVon Sigler, W.
000168750 700__ $$0241276$$g120925$$aHarms, Hauke
000168750 700__ $$aGremion, F.
000168750 773__ $$j48$$tFems Microbiology Ecology$$q273-283
000168750 8564_ $$uhttps://infoscience.epfl.ch/record/168750/files/48-2-273.pdf$$zPUBLISHER'S VERSION$$s230731
000168750 909C0 $$0252382$$pLPE
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000168750 917Z8 $$x173008
000168750 937__ $$aEPFL-ARTICLE-168750
000168750 973__ $$rREVIEWED$$sPUBLISHED$$aEPFL
000168750 980__ $$aARTICLE