Quantitative Photo Activated Localization Microscopy: Unraveling the Effects of Photoblinking

In this work we discuss how to use photophysical information for improved quantitative measurements using Photo Activated Localization Microscopy (PALM) imaging. We introduce a method that reliably estimates the number of photoblinking molecules present in a biological sample and gives a robust way to quantify proteins at the single-cell level from PALM images. We apply this method to determine the amount of beta 2 adrenergic receptor, a prototypical G Protein Coupled Receptor, expressed on the plasma membrane of HeLa cells.


Published in:
PLoS ONE, 6, 7, e22678
Year:
2011
Publisher:
Public Library of Science
ISSN:
1932-6203
Keywords:
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 Record created 2011-09-05, last modified 2018-12-03

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