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research article

A simple high-yielding process for transient gene expression in CHO cells

Rajendra, Yashas
•
Kiseljak, Divor
•
Baldi, Lucia  
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2011
Journal of Biotechnology

Here we describe a simplified method for transient gene expression (TGE) in suspension-adapted Chinese hamster ovary (CHO) cells using polyethylenimine (PEI) for DNA delivery. Both the transfection and production phases of the bioprocess were performed at a density of 4 × 106 cells/mL at 31 °C. In addition, the amounts of both PEI and plasmid DNA were reduced up to 50% on a per cell basis compared to previously published protocols from this laboratory, resulting in higher cell viability after transfection and higher volumetric recombinant protein yields. In batch cultures of up to 14 days, reproducible recombinant antibody yields up to 300 mg/L were achieved at small scale (5 mL) and up to 250 mg/L at large scale (500 mL). The simplicity and improved yields are expected to increase the utility of CHO cells for the rapid production of recombinant proteins at larger scales by TGE.

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Type
research article
DOI
10.1016/j.jbiotec.2011.03.001
Web of Science ID

WOS:000290208000004

Author(s)
Rajendra, Yashas
Kiseljak, Divor
Baldi, Lucia  
Hacker, David L.  
Wurm, Florian M.  
Date Issued

2011

Publisher

Elsevier

Published in
Journal of Biotechnology
Volume

153

Issue

1-2

Start page

22

End page

26

Subjects

Transient gene expression

•

Polyethylenimine

•

Hypothermia

•

Chinese hamster ovary cells

•

Orbital shaking

•

Antibody

Editorial or Peer reviewed

REVIEWED

Written at

EPFL

EPFL units
LBTC  
Available on Infoscience
August 22, 2011
Use this identifier to reference this record
https://infoscience.epfl.ch/handle/20.500.14299/70206
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