000167096 001__ 167096
000167096 005__ 20190316235147.0
000167096 0247_ $$2doi$$a10.1073/pnas.1016150108
000167096 022__ $$a1091-6490
000167096 02470 $$2ISI$$a000287844400034
000167096 037__ $$aARTICLE
000167096 245__ $$aTightening of DNA knots by supercoiling facilitates their unknotting by type II DNA topoisomerases
000167096 269__ $$a2011
000167096 260__ $$c2011
000167096 336__ $$aJournal Articles
000167096 520__ $$aUsing numerical simulations, we compare properties of knotted DNA molecules that are either torsionally relaxed or supercoiled. We observe that DNA supercoiling tightens knotted portions of DNA molecules and accentuates the difference in curvature between knotted and unknotted regions. The increased curvature of knotted regions is expected to make them preferential substrates of type IIA topoisomerases because various earlier experiments have concluded that type IIA DNA topoisomerases preferentially interact with highly curved DNA regions. The supercoiling-induced tightening of DNA knots observed here shows that torsional tension in DNA may serve to expose DNA knots to the unknotting action of type IIA topoisomerases, and thus explains how these topoisomerases could maintain a low knotting equilibrium in vivo, even for long DNA molecules.
000167096 6531_ $$aBROWNIAN DYNAMICS SIMULATION; ESCHERICHIA-COLI; PLASMID PBR322; WORMLIKE-CHAIN; TOPOLOGY; SIMPLIFICATION; CATENANES; MECHANISM; MOLECULE; GYRASE
000167096 700__ $$0240685$$g147867$$aWitz, G.
000167096 700__ $$g125942$$aDietler, G.$$0240091
000167096 700__ $$aStasiak, A.
000167096 773__ $$j108$$tProceedings of the National Academy of Sciences$$k9$$q3608-3611
000167096 8564_ $$uhttps://infoscience.epfl.ch/record/167096/files/PNAS-2011-Witz-3608-11.pdf$$zn/a$$s1824450$$yPublisher's version
000167096 909C0 $$xU10868$$0252162$$pLPMV
000167096 909CO $$qGLOBAL_SET$$pSB$$ooai:infoscience.tind.io:167096$$particle
000167096 917Z8 $$x105590
000167096 937__ $$aEPFL-ARTICLE-167096
000167096 973__ $$rREVIEWED$$sPUBLISHED$$aEPFL
000167096 980__ $$aARTICLE