000167054 001__ 167054
000167054 005__ 20180318102733.0
000167054 0247_ $$2doi$$a10.1117/12.889246
000167054 02470 $$2ISI$$a000293625400003
000167054 037__ $$aCONF
000167054 245__ $$aCell death detection and ionic homeostasis monitoring with digital holographic microscopy 
000167054 269__ $$a2011
000167054 260__ $$bSPIE$$c2011
000167054 336__ $$aConference Papers
000167054 490__ $$aProceedings of SPIE
000167054 520__ $$aDigital holographic microscopy is an interferometric technique enabling the measurement of the quantitative phase shifts induced by cell bodies. We correlate the phase signal measured on neurons with calcium imaging measured by fluorescence on cells loaded with Fluo-4, to monitor responses to glutamate challenges, which provoke well-known calcium increases through activation of various membrane receptors. A very good correspondence can be identified between the two signals, showing the links between the phase signal, being a measure of the intracellular dilution, and the calcium concentration within cells. We then check cell viability by employing propidium iodide (PI), a fluorescent indicator relying on the cell membrane integrity loss to assess cell death. Strong intracellular calcium concentration is indeed known to induce excitotoxic effects, potentially inducing cell death. This enables showing that some cells cannot sustain the calcium saturation identified in our measurements, leading to subsequent cell death.
000167054 6531_ $$a[MVD]
000167054 700__ $$0242050$$aPavillon, Nicolas$$g146896
000167054 700__ $$aKühn, Jonas
000167054 700__ $$0242052$$aJourdain, Pascal$$g159871
000167054 700__ $$0240009$$aDepeursinge, Christian$$g104931
000167054 700__ $$0243698$$aMagistretti, Pierre$$g134990
000167054 700__ $$aMarquet, Pierre
000167054 7112_ $$aEuropean Conferences on Biomedical Optics$$cMunich$$dMay 22-26, 2011
000167054 773__ $$j8090$$q809004$$tNovel Optical Instrumentation for Biomedical Applications V
000167054 8564_ $$uhttp://spiedigitallibrary.org/proceedings/resource/2/psisdg/8090/1/809004_1$$zURL
000167054 909CO $$ooai:infoscience.tind.io:167054$$pSV$$pconf$$pSTI
000167054 909C0 $$0252265$$pLNDC$$xU11150
000167054 909C0 $$0252008$$pLOA$$xU10346
000167054 917Z8 $$x146896
000167054 937__ $$aEPFL-CONF-167054
000167054 973__ $$aEPFL$$rNON-REVIEWED$$sPUBLISHED
000167054 980__ $$aCONF