000166627 001__ 166627
000166627 005__ 20181203022409.0
000166627 0247_ $$2doi$$a10.1021/nn1023285
000166627 022__ $$a1936-086X
000166627 02470 $$2ISI$$a000289742100020
000166627 037__ $$aARTICLE
000166627 245__ $$aDirect Investigation of Intracellular Presence of Gold Nanoparticles
000166627 260__ $$c2011
000166627 269__ $$a2011
000166627 336__ $$aJournal Articles
000166627 520__ $$aNanotechnology as well as advanced microscopy can play a fundamental role in understanding biological mechanisms. Here we present a study that combines a new type of nanomaterial with a new type of microscopy and highlights the potential for gathering novel Information about cell membrane penetration and cytosol local viscosity. On the material side, we used gold nanoparticles that have an ordered stripe-like arrangement of domains. These "striped" nanoparticles are able to penetrate cell membranes directly without porating them. On the microscopy side, we used photothermal heterodyne imaging which allows detection of individual nanometer-sized gold particles in complex media. We showed that we can probe cytosolic presence as well as dynamics of these nanoparticles even at very low concentrations. We used the fluctuations of the photothermal signal from particles diffusing In the detection volume to estimate local cytosol viscosity which is about 20 times larger than that of water. This work opens new perspectives for mapping local diffusion properties of nano-objects inside living cells.
000166627 6531_ $$agold nanoparticle
000166627 6531_ $$acell penetration
000166627 6531_ $$aphotothermal imaging
000166627 6531_ $$acytosol viscosity
000166627 6531_ $$aself-assembled monolayer
000166627 700__ $$aLeduc, CéCile
000166627 700__ $$aJung, Jin-Mi
000166627 700__ $$aCarney, Randy P.
000166627 700__ $$0244724$$g196998$$aStellacci, Francesco
000166627 700__ $$aLounis, Brahim
000166627 773__ $$j5$$tACS Nano$$k4$$q2587-2592
000166627 909C0 $$xU12173$$0252323$$pSUNMIL
000166627 909CO $$pSTI$$particle$$ooai:infoscience.tind.io:166627
000166627 917Z8 $$x204526
000166627 937__ $$aEPFL-ARTICLE-166627
000166627 973__ $$rREVIEWED$$sPUBLISHED$$aOTHER
000166627 980__ $$aARTICLE