000165657 001__ 165657
000165657 005__ 20180913060621.0
000165657 0247_ $$2doi$$a10.1002/9780470942390.mo100169
000165657 037__ $$aARTICLE
000165657 245__ $$aLentiviral Vector Mediated Transgenesis
000165657 269__ $$a2011
000165657 260__ $$c2011
000165657 336__ $$aJournal Articles
000165657 520__ $$aThe genetic manipulation of rodents through the generation of fully transgenic animals or via the modification of selective cells or organs is a procedure of paramount importance for biomedical research, either to address fundamental questions or to develop preclinical models of human diseases. Lentiviral vectors occupy the front stage in this scene, as they can mediate the integration and stable expression of transgenes both in vitro and in vivo. Widely used to modify a variety of cells, including re-implantable somatic and embryonic stem cells, lentiviral vectors can also be directly administered in vivo, for instance in the brain. However, perhaps their most spectacular research application is in the generation of transgenic animals. Compared with the three-decade-old DNA pronuclear injection technique, lentivector-mediated transgenesis is simple, cheap, and highly efficient. Furthermore, it can take full advantage of the great diversity of lentiviral vectors developed for other applications, and thus allows for ubiquitous or tissue-specific or constitutive or externally controllable transgene expression, as well as RNAi-mediated gene knockdown
000165657 6531_ $$alentiviral vector , transgenesis , transgenic animals
000165657 700__ $$0240844$$aBarde, Isabelle$$g168886
000165657 700__ $$aVerp, Sonia
000165657 700__ $$aOffner, Sandra Eloise
000165657 700__ $$0240083$$aTrono, Didier$$g167919
000165657 773__ $$j1$$q169-184$$tCurrent Protocols in Mouse Biology 1
000165657 909C0 $$0252036$$pLVG$$xU11172
000165657 909CO $$ooai:infoscience.tind.io:165657$$pSV$$particle
000165657 917Z8 $$x169414
000165657 937__ $$aEPFL-ARTICLE-165657
000165657 973__ $$aEPFL$$rNON-REVIEWED$$sPUBLISHED
000165657 980__ $$aARTICLE