Overcoming barriers to membrane protein structure determination
After decades of slow progress, the pace of research on membrane protein structures is beginning to quicken thanks to various improvements in technology, including protein engineering and microfocus X-ray diffraction. Here we review these developments and, where possible, highlight generic new approaches to solving membrane protein structures based on recent technological advances. Rational approaches to overcoming the bottlenecks in the field are urgently required as membrane proteins, which typically comprise similar to 30% of the proteomes of organisms, are dramatically under-represented in the structural database of the Protein Data Bank.
Keywords: Size-Exclusion Chromatography ; Hydantoin Transporter Mhp1 ; X-Ray Crystallography ; Cytochrome-C-Oxidase ; Crystal-Structure ; Coupled Receptor ; Escherichia-Coli ; Lactococcus-Lactis ; Molecular-Basis ; Angstrom Resolution
Record created on 2011-05-03, modified on 2016-08-09