000164840 001__ 164840
000164840 005__ 20190416055800.0
000164840 022__ $$a1094-4087
000164840 02470 $$2ISI$$a000235012000057
000164840 037__ $$aCONF
000164840 245__ $$aConfining the sampling volume for Fluorescence Correlation Spectroscopy using a sub-wavelength sized aperture
000164840 269__ $$a2006
000164840 260__ $$bOptical Society of America$$c2006
000164840 336__ $$aConference Papers
000164840 520__ $$aFor the observation of single molecule dynamics with fluorescence fluctuation spectroscopy (FFS) very low fluorophore concentrations are necessary. For in vitro measurements, this requirement is easy to fulfill. In biology however, micromolar concentrations are often encountered and may pose a real challenge to conventional FFS methods based on confocal instrumentation. We show a higher confinement of the sampling volume in the near-field of sub-wavelength sized apertures in a thin gold film. The gold apertures have been measured and characterized with fluorescence correlation spectroscopy (FCS), indicating light confinement beyond the far-field diffraction limit. We measured a reduction of the effective sampling volume by an order of magnitude compared to confocal instrumentation. (c) 2006 Optical Society of America.
000164840 6531_ $$aPhoton-Counting Histogram
000164840 6531_ $$aFluctuation Spectroscopy
000164840 6531_ $$aNano-Apertures
000164840 6531_ $$aMolecule
000164840 700__ $$aGosch, M.
000164840 700__ $$aPerentes, A.
000164840 700__ $$aHoffmann, P.
000164840 700__ $$0244723$$g159310$$aMartin, O. J. F.
000164840 700__ $$aLasser, T.
000164840 700__ $$aLeutenegger, M.
000164840 7112_ $$dJun, 2005$$cCharlotte, NC$$aOSA Topical Meeting on Signal Recovery and Synthesis
000164840 773__ $$j14$$tOptics Express$$q956-969
000164840 8564_ $$uhttps://infoscience.epfl.ch/record/164840/files/073.pdf$$zn/a$$s363395$$yn/a
000164840 909C0 $$xU10373$$0252353$$pNAM
000164840 909CO $$ooai:infoscience.tind.io:164840$$qGLOBAL_SET$$pconf$$pSTI
000164840 917Z8 $$x178738
000164840 937__ $$aEPFL-CONF-164840
000164840 973__ $$rREVIEWED$$sPUBLISHED$$aEPFL
000164840 980__ $$aCONF