Neural stem cells are increased after loss of β-catenin, but neural progenitors undergo cell death
Neurons and glia in the central nervous system originate from neural stem and progenitor cells that reside in the ventricular zones. Here we examine the role of beta-catenin in neural stem cell (NSC) regulation in mouse embryos lacking beta-catenin specifically in the brain germinal zone. An in vitro clonal neurosphere assay was performed in order to ascertain the status of the NSC population. Intact neurospheres did not form from beta-catenin-null cells due to a loss of cell adhesion and the number of expanded cells was reduced. Rescue of beta-catenin expression restored adhesion and revealed that the number of NSCs increased in the knockout population. Using a clonal colony-forming assay, which confines precursor cells within a solid collagen matrix, we show that the number of NSCs in the hippocampus is unchanged although the beta-catenin knockout striatum actually contains a larger proportion of NSCs. However, these colonies were smaller than those of control cells, due to increased apoptosis in the progenitor population. Furthermore, beta-catenin knockout NSCs also retained multipotentiality as shown by their ability to clonally differentiate into neurons and glia. The effects on neural precursor cells were not due to loss of downstream T-cell factor signaling, as this pathway is not active in vivo in regions of the embryonic brain where NSCs and progenitor cells reside, nor is it active in vitro in NSC colonies. These data reveal that beta-catenin is not required for the maintenance or differentiation of NSCs, but is required for the adhesion and survival of neural progenitor cells.
Keywords: cell adhesion ; cell survival ; mouse embryos ; neurosphere assay ; T-cell factor ; Wnt ; Wnt/Beta-Catenin ; Neuronal Differentiation ; Nervous-System ; Mouse Telencephalon ; Precursor Cells ; Gamma-Catenin ; Neurogenesis ; Hippocampus ; Proliferation ; Hematopoiesis
Record created on 2011-04-05, modified on 2016-08-09