000164357 001__ 164357
000164357 005__ 20181203022328.0
000164357 0247_ $$2doi$$a10.1038/emboj.2008.126
000164357 022__ $$a1460-2075
000164357 037__ $$aARTICLE
000164357 245__ $$aThe DEAD-box helicase DDX3X is a critical component of the TANK-binding kinase 1-dependent innate immune response
000164357 269__ $$a2008
000164357 260__ $$c2008
000164357 336__ $$aJournal Articles
000164357 520__ $$aTANK-binding kinase 1 (TBK1) is of central importance for the induction of type-I interferon (IFN) in response to pathogens. We identified the DEAD-box helicase DDX3X as an interaction partner of TBK1. TBK1 and DDX3X acted synergistically in their ability to stimulate the IFN promoter, whereas RNAi-mediated reduction of DDX3X expression led to an impairment of IFN production. Chromatin immunoprecipitation indicated that DDX3X is recruited to the IFN promoter upon infection with Listeria monocytogenes, suggesting a transcriptional mechanism of action. DDX3X was found to be a TBK1 substrate in vitro and in vivo. Phosphorylation-deficient mutants of DDX3X failed to synergize with TBK1 in their ability to stimulate the IFN promoter. Overall, our data imply that DDX3X is a critical effector of TBK1 that is necessary for type I IFN induction.
000164357 6531_ $$aImmunity, Innate
000164357 700__ $$aSoulat, Didier
000164357 700__ $$aBürckstümmer, Tilmann
000164357 700__ $$aWestermayer, Sandra
000164357 700__ $$aGoncalves, Adriana
000164357 700__ $$aBauch, Angela
000164357 700__ $$aStefanovic, Adrijana
000164357 700__ $$0245280$$g209845$$aHantschel, Oliver
000164357 700__ $$aBennett, Keiryn L.
000164357 700__ $$aDecker, Thomas
000164357 700__ $$aSuperti-Furga, Giulio
000164357 773__ $$j27$$tThe EMBO journal$$k15$$q2135-46
000164357 909C0 $$xU12375$$0252328$$pUPHAN
000164357 909CO $$pSV$$particle$$ooai:infoscience.tind.io:164357
000164357 917Z8 $$x182396
000164357 937__ $$aEPFL-ARTICLE-164357
000164357 973__ $$rREVIEWED$$sPUBLISHED$$aOTHER
000164357 980__ $$aARTICLE