000164201 001__ 164201
000164201 005__ 20190316235045.0
000164201 0247_ $$2doi$$a10.1039/c0nj00440e
000164201 022__ $$a1144-0546
000164201 02470 $$2ISI$$a000284544800029
000164201 037__ $$aARTICLE
000164201 245__ $$aIncreasing the efficiency of lanthanide luminescent bioprobes: bioconjugated silica nanoparticles as markers for cancerous cells
000164201 269__ $$a2010
000164201 260__ $$bRoyal Society of Chemistry$$c2010
000164201 336__ $$aJournal Articles
000164201 520__ $$aThe lanthanide binuclear helicate [Ln(2)(L-C2)(3)] has been embedded into bare and NH2-functionalized silica nanoparticles (NPs) using water-in-oil microemulsion technique. TEM analysis reveals both [Ln(2)(L-C2)(3)]@SiO2 and [Ln(2)(L-C2)(3)]@SiO2/NH2 nanoparticles having a spherical morphology and being monodispersed with an average size of 55 +/- 5 and 90 +/- 10 nm, respectively. The energy of the ligand triplet state, similar to 21 800 cm(-1) ([Gd-2(L-C2)(3)]@NP), does not change upon incorporation into silica nanoparticles and is optimal for sensitizing Eu-III luminescence. As a consequence, [Eu-2(L-C2)(3)]@SiO2 and [Eu-2(L-C2)(3)]@SiO2/NH2 NPs display red emission due to characteristic D-5(0) > F-7(J) (J = 0-4) transitions with absolute quantum yield reaching 28% for the latter. NH2-functionalized NPs have then been conjugated with avidin (NP-avidin) or goat anti-mouse IgG antibody (NP-IgG) to test them as luminescent biomarkers. Time-resolved microscopy of immunocytochemical assays involving recognition of mucin-like proteins expressed on breast cancer MCF-7 cells by the 5D10 monoclonal antibody confirms that the NP-IgG bioprobe displays specific luminescent signal with signal-to-noise ratio approximate to 20% higher than the one obtained for the bioconjugate of molecular [Eu-2(L-C2(COOH))(3)] with IgG. In addition, immunoassays using a streptavidin-coated plate and the NP-IgG probe are able to detect 15 ng mL(-1) of the biotinylated 5D10 antibody with a signal-to-noise ratio of 100.
000164201 6531_ $$aRadiative Lifetime
000164201 6531_ $$aFluorescence
000164201 6531_ $$aExcitation
000164201 6531_ $$aComplexes
000164201 6531_ $$aAntibody
000164201 6531_ $$aProbes
000164201 700__ $$0241991$$g188686$$aEliseeva, Svetlana V.
000164201 700__ $$0242690$$g176618$$aSong, Bo
000164201 700__ $$0240440$$g138412$$aVandevyver, Caroline D. B.
000164201 700__ $$g149479$$aChauvin, Anne-Sophie$$0240735
000164201 700__ $$aWacker, Josias B.
000164201 700__ $$aBuenzli, Jean-Claude G.$$0241990$$g122803
000164201 773__ $$j34$$tNew Journal Of Chemistry$$q2915-2921
000164201 8564_ $$uhttps://infoscience.epfl.ch/record/164201/files/NJC-10-Nanoparticles_SE.pdf$$zn/a$$s1499126$$yn/a
000164201 909C0 $$xU10099$$0252081$$pLCSL
000164201 909CO $$ooai:infoscience.tind.io:164201$$qGLOBAL_SET$$pSB$$particle
000164201 917Z8 $$x206725
000164201 937__ $$aEPFL-ARTICLE-164201
000164201 973__ $$rNON-REVIEWED$$sPUBLISHED$$aEPFL
000164201 980__ $$aARTICLE