A MART-1::Cre transgenic line induces recombination in melanocytes and RPE
The number of transgenic mouse lines expressing Cre in either type of pigment cells (melanocytes and retinal pigment epithelium, RPE) is limited, and the available lines do not always offer sufficient specificity. In this study, we addressed this issue and we report on the generation of a MART-1::Cre BAC transgenic mouse line, in which the expression of Cre recombinase is controlled by regulatory elements of the pigment cell-specific gene MART-1 (mlana). When MART-1::Cre BAC transgenic mice were bred with the ROSA26-R reporter line, ß-galactosidase expression was observed in RPE from E12.5 onwards, and in melanocyte precursors from E17.5, indicating that the MART-1::Cre line provides Cre recombinase activity in pigment-producing cells rather than in a particular lineage. In addition, breeding of this mouse line to mice carrying a conditional allele of RBP-Jκ corroborated the reported phenotypes in both pigment cell lineages, inducing hair greying and microphthalmia. Our results thus suggest, that the MART-1::Cre line may serve as a novel and useful tool for functional studies in melanocytes and the RPE. © 2011 Wiley-Liss, Inc.
Keywords: melanocyte ; Melan-A ; mlana ; conditional knockout ; Cre recombinase ; Notch ; RBP-J kappa ; retinal pigment epithelium ; transgenic ; Neural Crest Cells ; Subcellular-Localization ; Regulatory Elements ; Stem-Cells ; Expression ; Protein ; Mice ; Differentiation ; Notch ; Melanoblasts
Record created on 2011-02-11, modified on 2016-08-09