Engineering the micro electrode environment with microfluidics: A new approach for cell culture patterning or controlled chemical stimulation
By enhancing the environment of micro electrode arrays with microfluidic channels we cultured primary neuronal cells in patterned two- and three dimensional structures. Alternatively, the same microfluidic channels can be used to generate a local chemical stimulation over a two-dimensional neuronal cell culture. We demonstrate that a significantly different of spontaneous activity pattern was recorded from the same neuronal cell culture under perfusion of 4.5μM bicuculline at 20 nl/s and 200 nl/s.
Record created on 2011-01-14, modified on 2016-08-09