Engineering the micro electrode environment with microfluidics: A new approach for cell culture patterning or controlled chemical stimulation

By enhancing the environment of micro electrode arrays with microfluidic channels we cultured primary neuronal cells in patterned two- and three dimensional structures. Alternatively, the same microfluidic channels can be used to generate a local chemical stimulation over a two-dimensional neuronal cell culture. We demonstrate that a significantly different of spontaneous activity pattern was recorded from the same neuronal cell culture under perfusion of 4.5μM bicuculline at 20 nl/s and 200 nl/s.


Published in:
Proceedings of the 7th International Meeting on Substrate-Integrated Microelectrode Arrays, 1, 316-317
Presented at:
7th Int. Meeting on Substrate Integrated Microelectrode Arrays (MEA2010), Reutlingen, Germany, June 29- July 2, 2010
Year:
2010
Laboratories:


Note: The status of this file is: EPFL only


 Record created 2011-01-14, last modified 2018-03-17

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