Study of Spatio-Temporal Immunofluorescence on Bead Patterns in a Microfluidic Channel

We performed a direct immunoassay inside a microfluidic channel on patterned streptavidin-coated beads, which captured fluorescently-labeled biotin target molecules from a continuous flow. We arranged the beads in a dot array at the bottom of the channel and demonstrated their position- and flow rate-dependent fluorescence. As the target analyte gets gradually depleted from the flow when passing downstream the channel, the highest fluorescence intensity was observed on the most upstream positioned dot patterns. We propose a simple analytical convection model to explain this spatio-temporal fluorescence.


Editor(s):
Häfeli, Urs
Schütt, Wolfgang
Zborowski, Maciej
Published in:
AIP Conference Proceedings, 1311, 1, 198-203
Presented at:
8th International Conference on the Scientific and Clinical Applications of Magnetic Carriers, Rostock, Germany , May 25-29, 2010
Year:
2010
Publisher:
American Institute of Physics
ISSN:
0094-243X
ISBN:
978-0-7354-0866-1
Keywords:
Laboratories:




 Record created 2011-01-05, last modified 2018-03-17

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