Deconvolution of 3D fluorescence micrographs with automatic risk minimization

We investigate the problem of automatic tuning of a deconvolution algorithm for three-dimensional (3D) fluorescence microscopy; specifically, the selection of the regularization parameter lambda. For this, we consider a realistic noise model for data obtained from a CCD detector: Poisson photon-counting noise plus Gaussian read-out noise. Based on this model, we develop a new risk measure which unbiasedly estimates the original mean-squared-error of the deconvolved signal estimate. We then show how to use this risk estimate to optimize the regularization parameter for Tikhonov-type deconvolution algorithms. We present experimental results on simulated data and numerically demonstrate the validity of the proposed risk measure. We also present results for real 3D microscopy data.

Published in:
2008 Ieee International Symposium On Biomedical Imaging: From Nano To Macro, Vols 1-4, 732-735
Presented at:
5th IEEE International Symposium on Biomedical Imaging - From Nano to Macro, Paris, FRANCE, May 14-17, 2008
Ieee Service Center, 445 Hoes Lane, Po Box 1331, Piscataway, Nj 08855-1331 Usa

 Record created 2010-11-30, last modified 2018-03-17

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