Chemical tethering of motile bacteria to silicon surfaces
We chemically immobilized live, motile Escherichia coli on micrometer-scale, photocatalytically patterned silicon surfaces via amine- and carboxylic acid-based chemistries. Immobilization facilitated (i) controlled positioning; (ii) high resolution cell wall imaging via atomic force microscopy (AFM); and (iii) chemical analysis with time-of-flight-secondary ion mass spectrometry (ToF-SIMS). Spinning motion of tethered bacteria, captured with fast-acquisition video, proved microbe viability. We expect our protocols to open new experimental doors for basic and applied studies of microorganisms, from host-pathogen relationships, to microbial forensics and drug discovery, to biosensors and biofuel cell optimization.
Keywords: E. coli ; tethering ; patterning ; microarrays ; imaging ; host-pathogen response ; drug discovery ; biofuel cells ; Microbial Fuel-Cells ; Electricity-Generation ; Cryoelectron Microscopy ; Protein Adsorption ; Bacillus-Subtilis ; Quantum Dots ; Biosensors ; Growth ; Microorganisms ; Metabolites
Record created on 2010-11-30, modified on 2016-08-09