Fast-fluorescence dynamics in nonratiometric calcium indicators

The fluorescence decay of high-affinity nonratiometric Ca2+ indicator Oregon Green BAPTA-1 (OGB-1) is analyzed with unprecedented temporal resolution in the two-photon excitation regime. A triple exponential decay is shown to best fit the fluorescence dynamics of OGB-1. We provide a model for accurate measurements of the free Ca2+ concentration and dissociation constants of nonratiometric calcium indicators.


Published in:
Optics letters, 34, 3, 362-4
Year:
2009
Publisher:
Optical Society of America
ISSN:
0146-9592
Keywords:
Laboratories:




 Record created 2010-11-30, last modified 2018-12-03


Rate this document:

Rate this document:
1
2
3
 
(Not yet reviewed)